Oana Teodora APREUTESEI1, Carmen Elena TEBRENCU2, Elena IONESCU3
Abstract. Hippophae rhamnoides L. (sea buckthorn) is increasingly consumed worldwide as home remedies and used as raw material for the pharmaceutical and food industry. An important step in the quality control of H. rhamnoides is establishing the appropriate analytical method to identify the specific marker profile. This study aimed to establish a chemical fingerprint for extract from H. rhamnoides branches as a reference for quality control and explore chemical variation in derived herbal formulations using thin-layer chromatography (HPTLC). The HPTLC analysis evidenced chlorogenic acid (Rf 0.52), kaempferol (Rf 0.98), and other polyphenolic acids (Rf 0.16, 0.25, 0.31, 0.48). A characteristic phytochemical fingerprint was established for H. rhmanoides branches that can be further used for exploring phytochemical profile and could serve as a valuable tool in the quality control of herbal formulations derived from H. rhamnoides.
Keywords: Hippophae rhamnoides L., herbal formulations, HPTLC fingerprint, quality control
DOI https://doi.org/10.56082/annalsarsciphyschem.2024.2.19
1Stejarul Research Centre for Biological Sciences, National Institute of Research and Development for Biological Sciences, Piatra Neamt, Romania
2Academy of Romanian Scientists Research and Processing Centre for Medicial Plants PLANTAVOREL SA, Piatra Neamt, Romania
3Academy of Romanian Scientists, Research and processing Centre for Medicial Plants PLANTAVOREL SA, Piatra Neamt, Romania
PUBLISHED in Annals of the Academy of Romanian Scientists Series on Physics and Chemistry, Volume 9, No2