MORALS, MOLECULES AND MOTION: EXPLORING HUMAN ETHICAL CONSIDERATIONS AND MOLECULAR BIOLOGY METHODS IN ZEBRAFISH MODELS EXPOSED TO VIBRATION-INDUCED OXIDATIVE STRESS


Ahmed Adel Mansour KAMAR¹, Alin-Stelian CIOBÎCĂ3Ioannis MAVROUDIS4, Manuela PĂDURARIU²

Abstract. Vibration exposure represents a complex mechanical stressor capable of triggering oxidative imbalance, inflammation, and neurophysiological alteration. The zebrafish (Danio rerio), with its strong genetic homology to humans and experimental versatility, offers an ethically responsible and translational model for investigating these molecular effects. This review synthesizes current evidence on vibration-induced oxidative stress while highlighting the ethical frameworks that must accompany such investigations. Within the molecular domain, recent studies demonstrate that controlled vibration activates redox pathways involving mitochondrial dysfunction, reactive oxygen species generation, and Nrf2-mediated antioxidant responses. Advances in molecular assays and omics technologies now permit detailed mapping of these biochemical events at organismal resolution. Parallel to these scientific developments, the moral dimension of zebrafish experimentation demands adherence to the principles of Replacement, Reduction, and Refinement (3Rs), together with transparent welfare monitoring and humane endpoints. Integrating ethical responsibility with molecular precision defines a new paradigm in vibration research—one that unites biological insight with moral accountability and strengthens the translational bridge between laboratory discovery and human occupational health.

Keywords: Zebrafish, oxidative stress, vibration exposure, bioethics, ROS, Nrf2, molecular-biology methods, translational research.

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DOI         10.56082/annalsarscibio.2025.2.35

¹Medical Department Head, MD Orthopedics Specialist, GUPCO – Cairo office, Egypt; Medical Resident, Department of Orthopedics and Traumatology, Spitalul Clinic de Recuperare, Iaşi, Romania; PhD Candidate, Faculty of Biology, “Alexandru Ioan Cuza” University of Iaşi, Romania

2MD, „Socola” Psychiatry Institute, Iaşi, Romania

3Professor, PhD, Department of Research in Neurobiology, Faculty of Biology, “Alexandru Ioan Cuza” University; “Ioan Hăulică” Institute, “Apollonia” University of Iaşi, Romania

4Professor, MD, PhD, FRCP, MRCP Consultant Neurologist, Leeds teaching Hospitals, University of Leeds, UK, Expert in neurodegeneration, oxidative stress, and translational neuroscience, Aristotle University of Thessaloniki (AUTH), Greece

Corresponding author: Ahmed Adel Mansour KAMAR; e-mail: ahmed81kamar@gmail.com


PUBLISHED in Annals Academy of Romanian Scientists Series on Biological SciencesVolume 14 no 2, 2025