ORIGINAL ARTICLE
Prevalence and
Antimicrobial Susceptibility Pattern of Acinetobacter baumannii Complex
in Clinical Samples Among Patients at a Tertiary Care Hospital, Jaipur
Monika ACHARYA1, Ved Prakash
MAMORIA1,
Supyar KUMAWAT1, Richa SHARMA1*
1Department of
Microbiology, Mahatma Gandhi University of Medical Sciences & Technology,
Jaipur, Rajasthan, India
Correspondence to: Dr. Richa
Sharma, Associate Professor, Department of Microbiology, Mahatma Gandhi
University of Medical Sciences & Technology, Jaipur, Rajasthan, India; E-mail:
richa.phd.15@gmail.com
Abstract: Aims and objectives: Acinetobacter causes a wide
spectrum of infections, including nosocomial pneumonia, secondary meningitis,
surgical wound infections, skin and soft tissue infections, urinary tract
infections, bacteraemia, and transmission via the hands of hospital personnel.
The study aimed to determine the prevalence of Acinetobacter baumannii complex
isolates and the antimicrobial susceptibility pattern of isolated A. baumannii
complex. in clinical samples among patients at Mahatma Gandhi Medical College
and Hospital. Introduction: In recent decades, Acinetobacter baumannii (A.
baumannii) infections have also occurred outside the ICU or in trauma patients
after natural disasters, and they have even affected patients after
co-morbidities in the community. Materials and methods: All A.
baumannii complex isolates (non-repetitive) from different clinical samples
received in a clinical microbiology laboratory from inpatients and outpatients
at Mahatma Gandhi Medical College and Hospital, Jaipur, Rajasthan, were
included in the study. Routine microscopy of the samples was done. Gram‘s staining was done on all samples except urine. All
clinical samples were inoculated on blood agar and MacConkey agar and incubated
at 370 °C for 18–24 hours. Antimicrobial susceptibility testing of the
isolated A. baumannii complex was done by the VITEK2-AST Compact system. Results: Among 6483 samples, 157 (2.42%) A.
baumannii complex isolates were culture-positive, 68.37% were sterile, and
29.19% were other culture-positive. The maximum sensitivity of A. baumannii
isolates was seen to be Tigecycline (70%), followed by Minocyclin
(29.9%), while maximum resistance was observed for Piperacillin/Toazobactam (97%), followed by Imipenem, Meropenem (96.8%),
Ceftazidime (96%), Cefepime (91.7%), Cipropfloxacin
(88%), and Gentamycin (87%). Conclusion: Based on this study,
it could be concluded that, as antibiotic resistance increases, hardships will
be experienced in A. baumannii complex treatment unless the necessary
precautions are taken and new antibiotics are discovered. In order to prevent
the spreading of resistant Acinetobacter strains, infection control measures
should be taken, clinicians and laboratory workers should cooperate during
antibiotic use, and hospital hygienic rules should be observed.
Keywords: Acinetobacter baumannii, antibiotic, susceptibility,
resistance.
DOI 10.56082/annalsarscimed.2024.1.6
